Worldwide
interest in seeking alternative methods has necessitated the evaluation
of in vitro techniques. Many existing in vitro systems are
technically demanding and have hidden expenses. The VectraCell gas
permeable containers offer the simplicity of T flask production with MAb
yields comparable to ascites. The one time use GP container is a
self-contained unit and is not reliant on mechanical motors, shakers and
spinners. It requires minimal labour, optimises incubator space
and produces high quality MAb.
BETA
TEST SITE RESULTS
Several
core hybridoma and commercial antibody laboratories were provided
VectraCell kits for evaluation. A total of 30 different cell lines
provided by the independent facilities were tested. The
flexibility of the VectraCell system allowed researchers to modify
techniques and optimise cell lines for increased MAb production.
For further information please contact us.
Orders
may be placed by phone, fax or via the Internet:
Cat. No.
3525 - VectraCell Single-Use Bioreactors
1 x 1L -
contains a 1L gas permeable container, sampling site couplers, dialysis
tubing, dialyzing solute and other materials for MAb production and
isolation/concentration
4 x 1L -
contains four 1L gas permeable containers and sampling site couplers
1 x 3L -
contains a 3L gas permeable container and sampling site couplers
4 x 3L -
contains four 3L gas permeable containers and sampling site couplers
COMING
SOON
Antibiotics
for Gene Selection
from AG Scientific, Inc.When
researchers want to focus on a primary gene of interest, the use of
antibiotic-resistance genes is very effective.
Antibiotic-resistance genes are used as markers to identify the transfer
of other genetic material (DNA) from one organism to another.
What
is an antibiotic-resistance gene?
An
antibiotic-resistance gene, when used as a marker, produces a protein
that protects plants and organisms from a specific antibiotic.
When exposed to the antibiotic, cells that have not taken up the new
gene are unable to grow and thus die, while transformed cells continue
to grow.
How
are antibiotics used for gene selection?
When transformed
cells (cells with an antibiotic-resistance gene) and untransformed cells
are exposed to an antibiotic, such as G-418, Hyromycin B, Puromycin and
Mycophenolic acid, only the transformed cells survive and grow.
Researchers can therefore be confident that the surviving cells contain
the gene of their primary research interest.
What
are the advantages of A.G. Scientific Antibiotics?
As a leading OEM
supplier and the largest supplier of Hygromycin B worldwide, A.G.
Scientific provides:
-
Superior
purity and activity
-
Best
product retail and wholesale pricing
-
Multiple
product forms (to meet the research needs of customers)
-
Custom
product packaging
-
OEM
supplier leadership
A.G.
Scientific's dedication to being a full service biochemical supplier
also includes providing the most comprehensive packaging and labelling
services available in the industry. Our capabilities
include: liquid fills, powder fills lyophilization, sterile
packaging, crimp seals, safety canisters, customer labelling and custom
cardboard packaging. A.G. Scientific guarantees the highest
standard will be provided to every customer project. We pledge our
commitment to aiding our customers' specific research needs.
PRODUCT
OVERVIEW
HYGROMYCIN
B
An
aminoglycosidic antibiotic produced by Streptomyces hygroscopius.
It kills bacteria, fungi and higher eukaryotic cells by inhibiting
protein synthesis.
Used in Molecular Biology for the selection and maintenance of pro- and
eukaryotic cells transfected with the E. coli hygromycin resistance
gene, hph. The hph gene confers hygromycin-resistance to
cells expressing it.
G418
(Geneticin®)
An
aminoglycosidic antibiotic that inhibits prokaryotic protein synthesis.
Used in Molecular Biology for the selection and maintenance of
eukaryotic cells transfected with the neo/kan gene. This
gene allows production of aminoglycoside 3'-phosphotransferase, which
inactivates G418, Neomycin and Kanamycin by phosphorylation.
PUROMYCIN
Puromycin
allows the selection of cells expressing the pac gene. The
expression of pac gene confers puromycin resistance to
transfected cells. Puromycin selection works for for Gram-positive
bacteria and animal cells. Most commonly utilised for
Mammalian Cell Culture.
Puromycin is an aminonucleoside antibiotic produced by Streptomyces
alboniger. It specifically inhibits peptidyl transfer on both
prokaryotic and eukaryotic ribosomes. The antibiotic inhibits the growth
of Gram-positive bacteria and various animal and insect cells.
Fungi and Gram-negative are resistant due to the low permeability of the
antibiotic. Puromycin has been widely used as a basic tool for
studying protein synthesis but now is widely utilised for the selection
of cell types harbouring plasmids carrying puromycin resistance genes.
MYCOPHENOLIC
ACID
Antibiotic
useful for the selection of animal cells that express the E. coli
gene coding for XGPRT (xanthine guanine phosphoribosyltransferase).
Also immunosuppressive agent
Bio
VectraTM dcl VectraCell kits are an easy to use and cost
effective alternative to existing methods for the production of
monoclonal antibodies (MABs). The VectraCell system is based on
the use of CO2/O2 gas permeable (GP) containers
which provide a closed environment for increased cell viability and
reduced contamination risks.